IVA Prime is a web application that allows you to import plasmid files (.gb and .dna), and then easily generate primers for IVA cloning as you edit your plasmid file. The primers can be then easily exported to a read-to-order format and the altered plasmid file can be saved.
The primer design technique is originally based on the work by García-Nafría et al.: DOI: 10.1038/srep27459.
The source code for IVA Prime can be found in the GitHub repository.
Click on Import File to import either a .gb or .dna file. (Or click Try Demo File to import an empty pET-28a(+) vector.)
Left click at the position you would like to insert a sequence.
Right-click to open the context menu and select
.
Select a region to be deleted.
Right-click to open the context menu and select
.
Select a region to be mutated/replaced.
Right-click to open the context menu and select
or .
To subclone navigate to your region of interest and select it.
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