IVA Prime

Calculate melting temperatures

Primer 1 Tm = -- °C

Primer 2 Tm = -- °C

Primer overlap Tm = -- °C

Basic settings

Primer generation settings

Primer type

Asym.

Sym.

Appearance

Theme

Advanced settings

Tm calculator settings

Primer concentration

Algorithm

Melting temperature equation.

Salt concentration

Salt correction

Salt correction equation.

DMSO concentration

DMSO correction equation.

Primer generation settings

HR minimum length

HR target Tm

Subcloning HR target Tm

TBR target Tm

Maximum Tm for short insertions

Use GC clamp

Reset to default settings Update settings

Import File New File Demo File

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Welcome to IVA Prime!

IVA Prime is freely available to all users without any login requirement. Check out the About page for more info.

Amino acid sequence

Selected: 0 bp

Tm = °C