Basic Settings

Distribute insertion across primer pairs:

Theme:

Grid size (bases per row):

Advanced Settings

Primer concentration [nM]:

Calculator Algorithm:

Salt concentration [M]:

Salt correction equation:

DMSO concentration [M]:

Homologous region minimum length [nt]:

Homologous region TM [°C]:

Subcloning homologous region TM [°C]:

Template binding region target TM [°C]:

Upper bound short insertions TM [°C]:

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