IVA Prime

IVA Cloning Operations

Insert here

Delete selection

Mutate selection

Insert from linear fragment

Mark selection for subcloning

Unmark subcloning target

Subclone into selection

Subclone with insertion(s) into selection

Annotate selection

Delete feature annotation

Copy selection

Copy special

Copy reverse

(top strand, 3'->5')

Copy reverse complement

(bottom strand, 5'->3')

Copy complement

(bottom strand, 3'->5')

Translate

Begin translation at first START codon (5'->3')

Begin translation at first START codon (3'->5')

Translate selection (5'->3')

Translate selection (3'->5')

Edit plasmid

Flip plasmid

Set new plasmid origin

Calculate melting temperatures

Primer 1 Tm = -- °C

Primer 2 Tm = -- °C

Primer overlap Tm = -- °C

Basic settings

Primer generation settings

Primer type

Asym.

Sym.

Appearance

Theme

Advanced settings

Tm calculator settings

Primer concentration

Algorithm

Melting temperature equation.

Salt concentration

Salt correction

Salt correction equation.

DMSO concentration

DMSO correction equation.

Primer generation settings

HR minimum length

HR target Tm

Subcloning HR target Tm

TBR target Tm

Maximum Tm for short insertions

Reset to default settings Update settings

Import File New File Demo File

‹

›

IVA Prime uses cookies to store user preferences across sessions. No third-party cookies are used to track or profile users.

Click here for more details

Understood

Welcome to IVA Prime!

IVA Prime is freely available to all users without any login requirement. Check out the About page for more info.

Amino acid sequence

Selected: 0 bp

Tm = °C

Color formatHexRGBHSL

Open color pickerColor swatch